我有个微生物相对丰度数据,想对分组 group 进行检验分析,采用 adonis 和 anosim 进行。结果如下:
Permutation test for adonis under reduced model
Permutation: free
Number of permutations: 999
vegan::adonis2(formula = specmat ~ group, data = specmat.meta, by = NULL)
Df SumOfSqs R2 F Pr(>F)
Model 1 0.4066 0.06274 10.978 0.001 ***
Residual 164 6.0742 0.93726
Total 165 6.4808 1.00000
---
Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1
Call:
vegan::anosim(x = specmat, grouping = specmat.meta$group)
Dissimilarity: bray
ANOSIM statistic R: 0.06837
Significance: 0.001
Permutation: free
Number of permutations: 999
我的问题是, adonis2 的 R2 = 0.06274 和 anosim 的 R = 0.06837 ,这个值会不会太小了,不足以解释差异?
1 回答
单细胞分析打工难