1.看文献,bowtie2比对ChIP-seq数据,The multimapped reads were kept for TE analysis, but only the best alignment was reported for those reads; if more than one equivalent best alignment was found, then one random alignment was reported.这里说的如果多于1个best的时候,随机选一个,应该怎么设置呢,bowtie2的default可以实现这个功能吗?
2.对于双端测序,bowtie2比对的aligned concordantly的reads,跟samtools flagstats里报告的properly paired reads,是一个意思吗?
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