snakepipes_fastqc-multiqc

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about author

author: 赵华男 | ZHAO Hua-nan

email: hermanzhaozzzz@gmail.com

Zhihu | BLOG

doc

snakepipes_fastqc-multiqc is a standard quality control snakemake pipeline for NGS/HTS data

• input file: FASTQ file by NGS sequencing, Single-end (SE) Paired-end (PE) are supported.
• output file:
  • fastqc report
  • multiqc report
• requirement
  • raw FASTQ file must put in ../fastq directory
  • only the same sequencing type (SE or PE) can be assigned into the sample.json at once!
  • SE sequencing data must named its suffix -> _SE.fastq.gz
  • PE sequencing data must named its suffix -> _R1.fastq.gz and_R2.fastq.gz
  • run Jupyter notebook to abtain the config for snakemake -> sample.json
  • run Snakemake to abtain the QC results at directory -> ../qc
    • summary html for QC stat -> ../qc/multiqc/multiqc_report.html

env:

tree .
.
└── fastq


git clone https://github.com/hermanzhaozzzz/snakepipes_fastqc-multiqc.git
cd snakepipes_fastqc-multiqc


conda env create -f conda_env.yml
conda activate snakepipes_fastqc-multiqc

run

# run Jupyter notebook to abtain the config
# run this cmd
# or
# open notebook and run all cells
runipy step.01.GetFileName.ipynb
# dry run for test
snakemake -pr -j 10 -s step.02.Snakefile.py -n
# run calculation
snakemake -pr -j 10 -s step.02.Snakefile.py

project structure

tree -L 2 .
.
├── fastq
│   ├── CTCF_ChIP-seq_CTCF-AID_auxin2days_rep1_SE.fastq.gz
│   ├── CTCF_ChIP-seq_CTCF-AID_auxin2days_rep2_SE.fastq.gz
│   ├── CTCF_ChIP-seq_CTCF-AID_untreated_rep1_SE.fastq.gz
│   ├── CTCF_ChIP-seq_CTCF-AID_untreated_rep2_SE.fastq.gz
│   ├── CTCF_ChIP-seq_CTCF-AID_washoff2days_rep1_SE.fastq.gz
│   ├── CTCF_ChIP-seq_CTCF-AID_washoff2days_rep2_SE.fastq.gz
│   ├── Input_for_CTCF_ChIP-seq_CTCF-AID_auxin2days_rep1_SE.fastq.gz
│   ├── Input_for_CTCF_ChIP-seq_CTCF-AID_auxin2days_rep2_SE.fastq.gz
│   ├── Spike-in-antibody-only_ChIP-seq_CTCF-AID_untreated_rep1_SE.fastq.gz
│   └── Spike-in-antibody-only_ChIP-seq_CTCF-AID_untreated_rep2_SE.fastq.gz
└── snakepipes_fastqc-multiqc
    ├── README.md
    ├── samples.json
    ├── step.01.GetFileName.ipynb
    └── step.02.Snakefile.smk.py